| Gene | Ddah2 |
| Chromosome | 17 |
| Deletion Start | 35,060,168 |
| Deletion End | 35,061,928 |
| Deletion Size | 1,761 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,060,852 | 35,060,874 | CCTCTCCAAGTGGACCAATCATC |
| TUP | 35,060,879 | 35,060,904 | AGCTGAGATCGTGGCAGACACGTTCC |
| TUR | 35,060,923 | 35,060,942 | GCCGCACTCATTCTCCAAAC |
| TDF | 35,061,170 | 35,061,190 | GCGTGGTTTAGGGCACAGAAG |
| TDP | 35,061,192 | 35,061,215 | TGCCTAGCTCTGGGAATTGCCCAC |
| TDR | 35,061,249 | 35,061,273 | CCATCATTACTAGGGTGTCCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGCAGCTGAGGGTTAAGGG |
| SD | TATCCCTTTGTCCCATCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13469A-C5 | VGB6 | C57BL/6NTac | |
| 13469A-C11 | VGB6 | C57BL/6NTac | |
| 13469A-G6 | VGB6 | C57BL/6NTac | |
| 13469A-E11 | VGB6 | C57BL/6NTac | |
| 13469A-G11 | VGB6 | C57BL/6NTac | |
| 13469A-F10 | VGB6 | C57BL/6NTac | |
