| Gene | Ddt |
| Chromosome | 10 |
| Deletion Start | 75,773,320 |
| Deletion End | 75,771,360 |
| Deletion Size | 1,961 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,772,770 | 75,772,789 | GCGCCAGCTTCTTCAAGTTC |
| TUP | 75,772,747 | 75,772,769 | CTCACCGAGGAGCTGTCCCTGGA |
| TUR | 75,772,704 | 75,772,724 | CGCACAAATACGTTCCCTCAC |
| TDF | 75,771,656 | 75,771,678 | CGGTATAGCCATCACCTTTCTTC |
| TDP | 75,771,626 | 75,771,655 | TGACTTGTCTTTGGTGCAAGGATCATTAGC |
| TDR | 75,771,573 | 75,771,592 | GACAGGCCAGCCCATATCAG |
PCR Primers
| Primer | Sequence |
| SU | CGGTTCCCGTACTGAGTTTG |
| SD | GCGACATCCCACTCCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13470A-D5 | VGB6 | C57BL/6NTac | |
| 13470A-D9 | VGB6 | C57BL/6NTac | |
| 13470A-F8 | VGB6 | C57BL/6NTac | |
| 13470A-G8 | VGB6 | C57BL/6NTac | |
| 13470A-H9 | VGB6 | C57BL/6NTac | |
