| Gene | Dgkz |
| Chromosome | 2 |
| Deletion Start | 91,963,443 |
| Deletion End | 91,933,401 |
| Deletion Size | 30,043 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,963,170 | 91,963,192 | GGTAGTGTGCTTCGTCCTTAGTG |
| TUP | 91,963,138 | 91,963,166 | TGATAGTCTGCTCCTAACCCTTCTGCAGG |
| TUR | 91,963,091 | 91,963,110 | AGGGTCTCTGACTGCTGGTG |
| TDF | 91,934,239 | 91,934,258 | ACCTCATGCACCGTGACCAG |
| TDP | 91,934,216 | 91,934,235 | AGCCGCACGCTCTTGCACCA |
| TDR | 91,934,173 | 91,934,193 | CAGCAGATAGCGGACCACTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGCGACGTGGCTTGAGGC |
| SD | CAGAGAGCCCAAAGGATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13473A-A8 | VGB6 | C57BL/6NTac | |
| 13473A-C6 | VGB6 | C57BL/6NTac | |
| 13473A-D6 | VGB6 | C57BL/6NTac | |
| 13473A-E2 | VGB6 | C57BL/6NTac | |
