| Gene | Dixdc1 |
| Chromosome | 9 |
| Deletion Start | 50,727,842 |
| Deletion End | 50,701,925 |
| Deletion Size | 25,918 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,727,468 | 50,727,488 | CAGGGACTCTCCAGGGACATC |
| TUP | 50,727,442 | 50,727,467 | CTAGAGGGAAGCCCTGAACAGATGCG |
| TUR | 50,727,401 | 50,727,421 | TCCCGTGACTCATCCGCTTAG |
| TDF | 50,702,145 | 50,702,167 | TGGCTCCCTCTTTCATCTTCTTG |
| TDP | 50,702,122 | 50,702,143 | CGGCACTGCACACTGAATGGCA |
| TDR | 50,702,079 | 50,702,098 | AGCCCTTCAGGTCAGGTCAG |
PCR Primers
| Primer | Sequence |
| SU | GCAGTGTAGTGATTGCAGGC |
| SD | TTCCAGGCTGCATGCAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13475A-C10 | VGB6 | C57BL/6NTac | |
| 13475A-G5 | VGB6 | C57BL/6NTac | |
| 13475A-D7 | VGB6 | C57BL/6NTac | |
