| Gene | Dkkl1 |
| Chromosome | 7 |
| Deletion Start | 45,211,550 |
| Deletion End | 45,207,582 |
| Deletion Size | 3,969 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,211,296 | 45,211,313 | GATGGGCGAGTGGTTGTC |
| TUP | 45,211,269 | 45,211,292 | CATGTACGAAGCCCTGGGCCTGAC |
| TUR | 45,211,239 | 45,211,262 | TCCTGAGTGCTGGTGTAATTGTTG |
| TDF | 45,207,978 | 45,208,001 | AGCAGCAAGTGAGCTGTAATGGAG |
| TDP | 45,207,952 | 45,207,975 | TGAGATGGCCCAGTGGAGACCAAA |
| TDR | 45,207,888 | 45,207,909 | ACTTTGGGAACCTGGGAATGAG |
PCR Primers
| Primer | Sequence |
| SU | GAACGCTCCAGTAATTTGCC |
| SD | GGGTGGCTCAGCAATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13476A-A2 | VGB6 | C57BL/6NTac | |
| 13476A-H8 | VGB6 | C57BL/6NTac | |
| 13476A-C2 | VGB6 | C57BL/6NTac | |
| 13476A-D2 | VGB6 | C57BL/6NTac | |
| 13476A-E5 | VGB6 | C57BL/6NTac | |
| 13476A-B10 | VGB6 | C57BL/6NTac | |
