| Gene | Dleu7 |
| Chromosome | 14 |
| Deletion Start | 62,292,947 |
| Deletion End | 62,276,897 |
| Deletion Size | 16,051 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,292,482 | 62,292,501 | GTTGCAGTGCCTGGATGAAG |
| TUP | 62,292,456 | 62,292,479 | AATTGGGCCACTGGAGTCCAAGCA |
| TUR | 62,292,435 | 62,292,453 | CACAGCAGGTTGACCACAC |
| TDF | 62,277,039 | 62,277,060 | GAAGGACAGCAGTTTGACAGAG |
| TDP | 62,277,012 | 62,277,037 | CTTGAATGCCGCTCACCAGTGTCTGA |
| TDR | 62,276,965 | 62,276,986 | TGATGGGAGATTAGCAAGTGAC |
PCR Primers
| Primer | Sequence |
| SU | AAGAACCAGAGCTCAGCATC |
| SD | TCAGAAAGTCAACAGTAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13477A-C1 | VGB6 | C57BL/6NTac | |
| 13477A-C8 | VGB6 | C57BL/6NTac | |
| 13477A-E8 | VGB6 | C57BL/6NTac | |
| 13477A-F4 | VGB6 | C57BL/6NTac | |
| 13477A-G8 | VGB6 | C57BL/6NTac | |
| 13477A-G10 | VGB6 | C57BL/6NTac | |
