| Gene | Popdc3 |
| Chromosome | 10 |
| Deletion Start | 45,318,043 |
| Deletion End | 45,314,798 |
| Deletion Size | 3,246 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,315,192 | 45,315,215 | CTGAAGTGGTTAGTTTGGAGAAGG |
| TUP | 45,315,216 | 45,315,237 | AGCACTGCTACGCCATGCAGGG |
| TUR | 45,315,255 | 45,315,274 | TGAGATGAGCACGGAGAGTC |
| TDF | 45,317,810 | 45,317,831 | GGCAGTGACATTGCAGATAAAC |
| TDP | 45,317,832 | 45,317,858 | TCTATGCCTTGAACGACAGGGTGTACA |
| TDR | 45,317,913 | 45,317,934 | GGTGATCTGGACAAGTCTGGAG |
PCR Primers
| Primer | Sequence |
| SU | ACAACGGGAAGACAATTTGC |
| SD | TGCTTCCCACAACAACAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13479A-A3 | VGB6 | C57BL/6NTac | |
| 13479A-A9 | VGB6 | C57BL/6NTac | |
| 13479A-H9 | VGB6 | C57BL/6NTac | |
| 13479A-F6 | VGB6 | C57BL/6NTac | |
| 13479A-E6 | VGB6 | C57BL/6NTac | |
| 13479A-A12 | VGB6 | C57BL/6NTac | |
