| Gene | Dnajc9 |
| Chromosome | 14 |
| Deletion Start | 20,388,780 |
| Deletion End | 20,385,282 |
| Deletion Size | 3,499 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,387,880 | 20,387,899 | TCACAGCGACGGAAATAGAC |
| TUP | 20,387,854 | 20,387,879 | TGCTGCGATATGACTGCTTGACCTGC |
| TUR | 20,387,818 | 20,387,837 | GGGCTTTCTTCCTGGTCATC |
| TDF | 20,386,245 | 20,386,267 | CAGCTATCCAGCTCCAGAGAAAG |
| TDP | 20,386,212 | 20,386,239 | AAATCATTGCAGCTCACTAGGCTGCTGT |
| TDR | 20,386,172 | 20,386,193 | CAGGCACTTCAGGATCTTCATC |
PCR Primers
| Primer | Sequence |
| SU | TATCATTGAGCTGTGCAGGG |
| SD | TGACGTCTCCTACTGCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13480A-F3 | VGB6 | C57BL/6NTac | |
| 13480A-E3 | VGB6 | C57BL/6NTac | |
| 13480A-F7 | VGB6 | C57BL/6NTac | |
| 13480A-B3 | VGB6 | C57BL/6NTac | |
| 13480A-F12 | VGB6 | C57BL/6NTac | |
