Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

13482 Dpep1 Status: ES cell colonies screened / QC positives

Gene	Dpep1
Chromosome	8
Deletion Start	123,194,086
Deletion End	123,201,106
Deletion Size	7,021
Genome Build	38

MGI ID	MGI:94917
NCBI Gene ID	13479
BAC ID	264H18
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	123,194,767	123,194,787	TCACTTGCCCATTGGGTCATC
TUP	123,194,788	123,194,810	CCTCCAATGGCCCTTCCACCAGC
TUR	123,194,862	123,194,882	CCGAAGAAGCAAAGCCATCTC
TDF	123,200,335	123,200,357	CGCTTAGGACTCACACCTCTTAC
TDP	123,200,358	123,200,381	TCCCTACAGACCACCTGGACCACA
TDR	123,200,383	123,200,401	AGCGCCTGCCACCTTCTTG

PCR Primers

Primer	Sequence
SU	GCCAGATGATAAGCAAACTGC
SD	TGGTACATGCACAGTGAGGG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	116 bp	+	+	-
TDF	TDR	67 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
13482A-D2	VGB6	C57BL/6NTac
13482A-E6	VGB6	C57BL/6NTac
13482A-F4	VGB6	C57BL/6NTac
13482A-H10	VGB6	C57BL/6NTac