| Gene | Drd4 |
| Chromosome | 7 |
| Deletion Start | 141,292,041 |
| Deletion End | 141,294,891 |
| Deletion Size | 2,851 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,292,914 | 141,292,933 | AAAGGGAGTGGGTTCAGGAC |
| TUP | 141,292,936 | 141,292,962 | TTTGGAGACTCCTGGAACACAGACACC |
| TUR | 141,292,989 | 141,293,009 | TTGCCAGTAACCACAGCAGAC |
| TDF | 141,294,095 | 141,294,114 | CGTCCGTCTGCTCCTTCTTC |
| TDP | 141,294,116 | 141,294,137 | TGCCCTGTCCGCTCATGCTACT |
| TDR | 141,294,139 | 141,294,159 | GCGGAAAGTGGCCCAGTAAAG |
PCR Primers
| Primer | Sequence |
| SU | CGTTAGTGAAAGAGGCGAGG |
| SD | CTCCAAGGAGCAAGAGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13486B-A1 | VGB6 | C57BL/6NTac | |
| 13486B-A4 | VGB6 | C57BL/6NTac | |
| 13486B-B12 | VGB6 | C57BL/6NTac | |
| 13486B-C3 | VGB6 | C57BL/6NTac | |
| 13486B-D10 | VGB6 | C57BL/6NTac | |
| 13486B-F9 | VGB6 | C57BL/6NTac | |
