| Gene | Ppp1r27 |
| Chromosome | 11 |
| Deletion Start | 120,551,050 |
| Deletion End | 120,550,179 |
| Deletion Size | 872 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,550,904 | 120,550,923 | AGCAGGTGGGACGCTTTATC |
| TUP | 120,550,879 | 120,550,903 | CGGGCTCGGAAAGTCTCCCTAGACA |
| TUR | 120,550,835 | 120,550,856 | GAAGGCCAGACTCTCAGTTATC |
| TDF | 120,550,259 | 120,550,280 | GATCTGCCCTCCGACCTTATTG |
| TDP | 120,550,232 | 120,550,258 | ACCCGGACTTCAAAGACTTGGTGGAAC |
| TDR | 120,550,207 | 120,550,227 | CAGTCCATGCTGGTTCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | CTTGAGCTCACATCCACAGC |
| SD | TCTCTGAACAAGAACATCGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13487A-E5 | VGB6 | C57BL/6NTac | |
| 13487A-F11 | VGB6 | C57BL/6NTac | |
