13488   E130012A19Rik     Status: ES cell colonies screened / QC positives

GeneE130012A19Rik
Chromosome11
Deletion Start97,629,278
Deletion End97,628,172
Deletion Size1,107
Genome Build38
MGI IDMGI:2143991
NCBI Gene ID103551
BAC ID270D16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,628,88197,628,899ACTTCGGCGCTGGTTCCTG
TUP97,628,85897,628,877ACCTCCGGGCCGCTGCCCTG
TUR97,628,82697,628,845GTTGGTCGGAGAGTCCTGAG
TDF97,628,56897,628,588CTCCGCCAGGAACATTTCAAC
TDP97,628,54197,628,565CCTGATCCGCCGCTCGAAACTTTGG
TDR97,628,49897,628,516GGCGCAAACTTGGAGTGTC

PCR Primers

PrimerSequence
SUAGAAAGAAGCTGAGAGGGCG
SDAAACAACCGGTTTGTCCATC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR74 bp++-
TDFTDR91 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13488C-F1VGB6C57BL/6NTac
13488D-D10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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