| Gene | Egr1 |
| Chromosome | 18 |
| Deletion Start | 34,861,492 |
| Deletion End | 34,863,777 |
| Deletion Size | 2,286 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,861,726 | 34,861,747 | AGCAGCGCCTTCAATCCTCAAG |
| TUP | 34,861,751 | 34,861,775 | AGCCGAGCGAACAACCCTATGAGCA |
| TUR | 34,861,782 | 34,861,801 | CAGACCACCGCTTACCTGTG |
| TDF | 34,863,009 | 34,863,029 | GCCTTCGCTCACTCCACTATC |
| TDP | 34,863,034 | 34,863,059 | ATTAAAGCCTTCGCCACTCAGTCGGG |
| TDR | 34,863,077 | 34,863,101 | TGGGATTGGTAGGTGGTATTAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTCGCGAGAGATCCCAG |
| SD | TGCTGTACAAAGATGCAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13489B-A3 | VGB6 | C57BL/6NTac | |
| 13489B-A8 | VGB6 | C57BL/6NTac | |
| 13489B-G10 | VGB6 | C57BL/6NTac | |
| 13489B-D11 | VGB6 | C57BL/6NTac | |
| 13489B-E2 | VGB6 | C57BL/6NTac | |
| 13489B-C5 | VGB6 | C57BL/6NTac | |
