| Gene | Elof1 |
| Chromosome | 9 |
| Deletion Start | 22,113,920 |
| Deletion End | 22,113,492 |
| Deletion Size | 429 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,113,859 | 22,113,877 | TGACAGGCACCCTGGAGAC |
| TUP | 22,113,836 | 22,113,858 | CCAGTTCACCTGCCCTTTCTGCA |
| TUR | 22,113,781 | 22,113,799 | GGATGCCCACCCTGAACAC |
| TDF | 22,113,606 | 22,113,625 | CCAGACACCCATCACATACC |
| TDP | 22,113,579 | 22,113,605 | TGTCAGAACCAGTGGATGTGTACAGCG |
| TDR | 22,113,524 | 22,113,543 | GGAGCAGGTCCCTGACTTTG |
PCR Primers
| Primer | Sequence |
| SU | GGCTGTGTTGTCATGGTTTG |
| SD | AAAGTCTAGGTCCCAAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13491A-D4 | VGB6 | C57BL/6NTac | |
| 13491A-E1 | VGB6 | C57BL/6NTac | |
| 13491A-E8 | VGB6 | C57BL/6NTac | |
| 13491A-H3 | VGB6 | C57BL/6NTac | |
| 13491A-G1 | VGB6 | C57BL/6NTac | |
| 13491A-B6 | VGB6 | C57BL/6NTac | |
