| Gene | Esrra |
| Chromosome | 19 |
| Deletion Start | 6,920,384 |
| Deletion End | 6,911,713 |
| Deletion Size | 8,672 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,920,115 | 6,920,133 | GGCCTCTGGCTACCACTAC |
| TUP | 6,920,087 | 6,920,110 | TGGCATCCTGTGAGGCCTGCAAAG |
| TUR | 6,920,060 | 6,920,079 | CACCCTGGATGGTCCTCTTG |
| TDF | 6,912,123 | 6,912,144 | GAGGTGTCCACTGGTGAGAAAG |
| TDP | 6,912,096 | 6,912,114 | CCGGCTCACAGGCAGAGCA |
| TDR | 6,912,067 | 6,912,089 | GGATACTCTGGGTGGGCTAATAC |
PCR Primers
| Primer | Sequence |
| SU | AGGATCATGCTTATGCTCGG |
| SD | CGGAGAGGAGATCGGATTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13493A-C5 | VGB6 | C57BL/6NTac | |
| 13493A-G5 | VGB6 | C57BL/6NTac | |
| 13493A-C9 | VGB6 | C57BL/6NTac | |
| 13493A-G4 | VGB6 | C57BL/6NTac | |
| 13493A-H6 | VGB6 | C57BL/6NTac | |
