| Gene | Etv2 |
| Chromosome | 7 |
| Deletion Start | 30,635,827 |
| Deletion End | 30,633,557 |
| Deletion Size | 2,271 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,635,748 | 30,635,767 | GGACTGGGTAAGCTGCTGTG |
| TUP | 30,635,719 | 30,635,746 | CTTGGGACAAAGTGTATGCAAGGCTCCA |
| TUR | 30,635,687 | 30,635,705 | GTCCCGGCTAGGTGTCAAG |
| TDF | 30,634,302 | 30,634,324 | GAAACTGGTTCTTGGGTCTCTAC |
| TDP | 30,634,271 | 30,634,296 | AGGGATCCAAACTCACAGACTCCCGA |
| TDR | 30,634,247 | 30,634,266 | CCAGGAGTCCAGCTCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGTGCATGGTATGAAATGGG |
| SD | TCCCTCCTGGGTCCTCTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13494A-A12 | VGB6 | C57BL/6NTac | |
| 13494A-H12 | VGB6 | C57BL/6NTac | |
| 13494A-D9 | VGB6 | C57BL/6NTac | |
| 13494A-H7 | VGB6 | C57BL/6NTac | |
| 13494A-F9 | VGB6 | C57BL/6NTac | |
| 13494A-E4 | VGB6 | C57BL/6NTac | |
