| Gene | Fastk |
| Chromosome | 5 |
| Deletion Start | 24,445,144 |
| Deletion End | 24,441,152 |
| Deletion Size | 3,993 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,444,641 | 24,444,662 | GACCACTAGCCCTGTGAATGAC |
| TUP | 24,444,617 | 24,444,640 | CTCAGGCTTAACCTCTCCGCCGCT |
| TUR | 24,444,554 | 24,444,573 | GTGCAAGCTGATGAGGAGTC |
| TDF | 24,441,546 | 24,441,569 | CCAGGCAAACTTTAACTCCACAAC |
| TDP | 24,441,521 | 24,441,544 | CAAGACCCTGCCCAAAGGTAGGGA |
| TDR | 24,441,469 | 24,441,488 | GGCTCAGGCTGCTGTTAGAC |
PCR Primers
| Primer | Sequence |
| SU | ATTGGAAACTGGTGGACTGG |
| SD | GCACAGCAGAGGGACTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13496A-F11 | VGB6 | C57BL/6NTac | |
| 13496A-B1 | VGB6 | C57BL/6NTac | |
| 13496A-C2 | VGB6 | C57BL/6NTac | |
| 13496A-A3 | VGB6 | C57BL/6NTac | |
