| Gene | Fau |
| Chromosome | 19 |
| Deletion Start | 6,058,254 |
| Deletion End | 6,059,487 |
| Deletion Size | 1,234 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,058,308 | 6,058,326 | ACGGTCGCCCAGATCAAAG |
| TUP | 6,058,327 | 6,058,350 | TAAGTATGTACTGATCCCTGATTC |
| TUR | 6,058,351 | 6,058,373 | TGGAGGTAAAGGAGTGCAGTTAG |
| TDF | 6,059,065 | 6,059,086 | TCAAGAGTTGAGGGAGGAAGAG |
| TDP | 6,059,087 | 6,059,109 | TTGCTCTGGGCGTGACCTTGCTA |
| TDR | 6,059,149 | 6,059,166 | AGCCAGGGAACCGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GTACAAACCACCAGCGATCC |
| SD | TGGGTGATCTGCCTTAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13498A-F9 | VGB6 | C57BL/6NTac | |
| 13498A-D2 | VGB6 | C57BL/6NTac | |
| 13498A-B5 | VGB6 | C57BL/6NTac | |
| 13498A-A10 | VGB6 | C57BL/6NTac | |
| 13498A-G9 | VGB6 | C57BL/6NTac | |
