| Gene | Fbxw5 |
| Chromosome | 2 |
| Deletion Start | 25,501,962 |
| Deletion End | 25,505,022 |
| Deletion Size | 3,061 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,502,812 | 25,502,831 | TTTGCTGTGCTGCCAGAGTG |
| TUP | 25,502,843 | 25,502,871 | TAATTACCCTGCAGCCTTTCTGGAACAGA |
| TUR | 25,502,876 | 25,502,897 | TGAGATGGTCAGGTCGTTGTTC |
| TDF | 25,504,740 | 25,504,760 | GGACCGCCACTACAACATCTG |
| TDP | 25,504,761 | 25,504,781 | CCTAGCCAAGCTGCGGCATGA |
| TDR | 25,504,783 | 25,504,803 | AGCCACGGAGTTGACCACATC |
PCR Primers
| Primer | Sequence |
| SU | CAAGTCCTTTGGTGTCCTGG |
| SD | CCAACTGTTTATTCAGGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13499A-C3 | VGB6 | C57BL/6NTac | |
| 13499A-G2 | VGB6 | C57BL/6NTac | |
| 13499A-F12 | VGB6 | C57BL/6NTac | |
| 13499A-H1 | VGB6 | C57BL/6NTac | |
| 13499A-F7 | VGB6 | C57BL/6NTac | |
