| Gene | Fgf15 |
| Chromosome | 7 |
| Deletion Start | 144,896,682 |
| Deletion End | 144,899,949 |
| Deletion Size | 3,268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,896,857 | 144,896,879 | CCTATGTCTCCAACTGCTTCCTC |
| TUP | 144,896,880 | 144,896,900 | CGAATCCGGAGCGACGGCTCT |
| TUR | 144,896,934 | 144,896,954 | GGGACGGGCTGAATGATACTC |
| TDF | 144,899,652 | 144,899,671 | CGCTACTCGGAGGAAGACTG |
| TDP | 144,899,674 | 144,899,702 | CCTTCAGGGAGGAAATGGACTGTTTAGGC |
| TDR | 144,899,741 | 144,899,761 | GGCTTGGCCTGGATGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | GTGCATTGAACACTTGCTCC |
| SD | CATACCTTCTGGCTTCCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13500A-F7 | VGB6 | C57BL/6NTac | |
| 13500A-G3 | VGB6 | C57BL/6NTac | |
