| Gene | Fkbp11 |
| Chromosome | 15 |
| Deletion Start | 98,728,082 |
| Deletion End | 98,724,356 |
| Deletion Size | 3,727 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,727,986 | 98,728,006 | CTGGGCCAGAAACCGAAAGTC |
| TUP | 98,727,959 | 98,727,980 | CGGACCCTCCAAGTGGAGACCC |
| TUR | 98,727,935 | 98,727,954 | AGTGGCACCACAGTCTCCTC |
| TDF | 98,724,574 | 98,724,594 | GTGGTGCAGTACGATGTGGAG |
| TDP | 98,724,543 | 98,724,569 | TCGCTTTGATTCGAGCCAACTACTGGC |
| TDR | 98,724,499 | 98,724,518 | CATGGCGATGCCTACCAGAG |
PCR Primers
| Primer | Sequence |
| SU | AGTGTGTGCCTCCCTTTCC |
| SD | GACAGCGAGAGAGGCAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13501A-E3 | VGB6 | C57BL/6NTac | |
| 13501A-G5 | VGB6 | C57BL/6NTac | |
