| Gene | Flii |
| Chromosome | 11 |
| Deletion Start | 60,727,186 |
| Deletion End | 60,714,247 |
| Deletion Size | 12,940 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,726,353 | 60,726,370 | TCACCTGCACACGGACAC |
| TUP | 60,726,327 | 60,726,349 | TTGGATCCCGTACACTGTGGGCA |
| TUR | 60,726,300 | 60,726,323 | GGTGGGTATGTAGACAGGATGAAG |
| TDF | 60,714,659 | 60,714,682 | GTGACCATTTCATCCTCTGTATCC |
| TDP | 60,714,633 | 60,714,658 | TCAGGTCTACATGTGGGTTGGGACCC |
| TDR | 60,714,588 | 60,714,607 | CCTGGCAAGCCTTCAGACTC |
PCR Primers
| Primer | Sequence |
| SU | CTGACGTTGTTACCACCATC |
| SD | TTCTAACGCCTGGACTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13503B-B9 | VGB6 | C57BL/6NTac | |
| 13503C-C2 | VGB6 | C57BL/6NTac | |
| 13503C-C7 | VGB6 | C57BL/6NTac | |
| 13503C-G6 | VGB6 | C57BL/6NTac | |
| 13503C-G9 | VGB6 | C57BL/6NTac | |
| 13503C-H10 | VGB6 | C57BL/6NTac | |
