| Gene | Fnbp1l |
| Chromosome | 3 |
| Deletion Start | 122,560,928 |
| Deletion End | 122,542,013 |
| Deletion Size | 18,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,560,070 | 122,560,089 | CAGAGCCTGTGACGACTTTC |
| TUP | 122,560,048 | 122,560,069 | TCCAACAGGGCCACACCTCCTC |
| TUR | 122,560,016 | 122,560,036 | AGGGAGCTGCACTATTAGGTG |
| TDF | 122,542,211 | 122,542,231 | TGAGGAGGACAAAGGTGATGG |
| TDP | 122,542,183 | 122,542,210 | ATGGACAAGAGCTCGGAGACAGAACGGT |
| TDR | 122,542,162 | 122,542,182 | TGTGGGAACGTAGCCTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATACTCCTTTGCCCTTGTGC |
| SD | GAACCTGAGCTGTCAGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13504A-E7 | VGB6 | C57BL/6NTac | |
| 13504A-G2 | VGB6 | C57BL/6NTac | |
| 13504A-H3 | VGB6 | C57BL/6NTac | |
| 13504A-H6 | VGB6 | C57BL/6NTac | |
