| Gene | Fosl1 |
| Chromosome | 19 |
| Deletion Start | 5,447,954 |
| Deletion End | 5,455,319 |
| Deletion Size | 7,366 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,448,252 | 5,448,272 | AGCGGGAAGCGCGAATATCTG |
| TUP | 5,448,282 | 5,448,307 | CTTCTCGCTCGCCCGTATCTCCCTAG |
| TUR | 5,448,330 | 5,448,354 | GGAAATGTAAGACCGTAACGGAGAC |
| TDF | 5,454,379 | 5,454,399 | GCCTCATTGTCTCCCTTCTTC |
| TDP | 5,454,402 | 5,454,426 | TCCCAACCTAGATCAGCCCAGAGGA |
| TDR | 5,454,445 | 5,454,463 | CTTGTTCCGCTCGCGTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGGGAACCTTGGCTAGTCTG |
| SD | GGGCACCTTCTGTCTAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13505A-A4 | VGB6 | C57BL/6NTac | |
| 13505A-D8 | VGB6 | C57BL/6NTac | |
| 13505A-F10 | VGB6 | C57BL/6NTac | |
| 13505A-G5 | VGB6 | C57BL/6NTac | |
| 13505A-H3 | VGB6 | C57BL/6NTac | |
