| Gene | Foxo3 |
| Chromosome | 10 |
| Deletion Start | 42,275,430 |
| Deletion End | 42,274,822 |
| Deletion Size | 609 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,275,388 | 42,275,405 | CGCTCTCTCCGCTCGAAG |
| TUP | 42,275,366 | 42,275,387 | TGGAGCTGGACCCAGAGTTCGA |
| TUR | 42,275,346 | 42,275,363 | AGCGTGGCCGACTCTGTG |
| TDF | 42,274,942 | 42,274,960 | CCTGTCCTATGCCGACCTG |
| TDP | 42,274,920 | 42,274,940 | TCACCCGCGCCATCGAGAGCT |
| TDR | 42,274,870 | 42,274,888 | GCGCACCATCCACTCGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCTAGTCCGGGAGAGACCTG |
| SD | TTGGTCCTTCCTCCTTAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13508A-C5 | VGB6 | C57BL/6NTac | |
| 13508B-F5 | VGB6 | C57BL/6NTac | |
