| Gene | Pgap2 |
| Chromosome | 7 |
| Deletion Start | 102,226,284 |
| Deletion End | 102,237,608 |
| Deletion Size | 11,325 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,227,124 | 102,227,143 | CTGTTGTGCTCCTCCTTCAC |
| TUP | 102,227,144 | 102,227,168 | TGATCGACAGTGCTTCCTGGGACCT |
| TUR | 102,227,230 | 102,227,252 | CCTGGACTCTACCCTCGTCTTAG |
| TDF | 102,237,147 | 102,237,169 | AGCGGCTCTTCGTCATCAACTTC |
| TDP | 102,237,172 | 102,237,193 | CTCCTTCTTCTCGGCGCTGGCT |
| TDR | 102,237,214 | 102,237,234 | TCACCTCCAGCCTCACAATAC |
PCR Primers
| Primer | Sequence |
| SU | AGCAGTGAGAACCAAGAGGC |
| SD | ACACCTGGGCAAATTTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 129 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13510A-D4 | VGB6 | C57BL/6NTac | |
| 13510B-C1 | VGB6 | C57BL/6NTac | |
| 13510B-G8 | VGB6 | C57BL/6NTac | |
| 13510C-F2 | VGB6 | C57BL/6NTac | |
