| Gene | Ftsj2 |
| Chromosome | 5 |
| Deletion Start | 140,331,857 |
| Deletion End | 140,328,291 |
| Deletion Size | 3,567 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,331,089 | 140,331,110 | TGCCTTCAAACTCCTGGAGATG |
| TUP | 140,331,059 | 140,331,084 | AGAAGCATCAGATCCTGAGGCCCGGT |
| TUR | 140,331,002 | 140,331,020 | CTGCACTGCCACCTGACTC |
| TDF | 140,328,631 | 140,328,649 | CCCAGCAGGAGAGCAGATG |
| TDP | 140,328,602 | 140,328,627 | TTCTGAGTGACATGGCACCGAATGCC |
| TDR | 140,328,550 | 140,328,571 | GGGTAAGGCACAAGCTGATGAG |
PCR Primers
| Primer | Sequence |
| SU | AATGCACATGGAGTGCAGAG |
| SD | ATTGCCAAAAACTAACAGTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13512A-A8 | VGB6 | C57BL/6NTac | |
| 13512A-G6 | VGB6 | C57BL/6NTac | |
| 13512A-D9 | VGB6 | C57BL/6NTac | |
| 13512A-E5 | VGB6 | C57BL/6NTac | |
| 13512A-G2 | VGB6 | C57BL/6NTac | |
| 13512A-F3 | VGB6 | C57BL/6NTac | |
