| Gene | Fut11 |
| Chromosome | 14 |
| Deletion Start | 20,695,006 |
| Deletion End | 20,698,414 |
| Deletion Size | 3,409 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,695,208 | 20,695,227 | TGGACCTACCAGTGTTGCTG |
| TUP | 20,695,228 | 20,695,250 | TGGTGGAGCCCAGGTCTGTTTCC |
| TUR | 20,695,252 | 20,695,272 | CTCCGAGTCACCTGGGAAGTG |
| TDF | 20,697,840 | 20,697,861 | GCTGCTTGGGAGAACTATGATG |
| TDP | 20,697,870 | 20,697,899 | TTTGTGACCACCTTCCTATCTCTTGGATGA |
| TDR | 20,697,914 | 20,697,936 | TGCTGTGAAGGAAAGAAGATGTG |
PCR Primers
| Primer | Sequence |
| SU | CAGGACAAGGGCTAGGAATG |
| SD | ACCCACACATAAGGCTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13513A-B9 | VGB6 | C57BL/6NTac | |
| 13513A-E10 | VGB6 | C57BL/6NTac | |
| 13513A-G8 | VGB6 | C57BL/6NTac | |
| 13513A-F3 | VGB6 | C57BL/6NTac | |
| 13513A-E11 | VGB6 | C57BL/6NTac | |
