| Gene | Gadd45b |
| Chromosome | 10 |
| Deletion Start | 80,930,319 |
| Deletion End | 80,931,744 |
| Deletion Size | 1,426 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,930,343 | 80,930,362 | GACAACGCGGTTCAGAAGTG |
| TUP | 80,930,367 | 80,930,391 | CAACAGAGGCTGAGGTCTTCTCGCC |
| TUR | 80,930,412 | 80,930,432 | GGGAGATTAGCCGATGACAGC |
| TDF | 80,931,034 | 80,931,052 | CCGACAGCGTGGTCTTGTG |
| TDP | 80,931,053 | 80,931,079 | CCTCCTGGCCATAGACGAAGAAGAGGA |
| TDR | 80,931,102 | 80,931,122 | CGACTGGATCAGGGTGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCATCTCCAGCCAATCTC |
| SD | TGTGCTGTAGCTGCGAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13515A-B1 | VGB6 | C57BL/6NTac | |
| 13515A-D11 | VGB6 | C57BL/6NTac | |
| 13515A-E3 | VGB6 | C57BL/6NTac | |
| 13515A-E5 | VGB6 | C57BL/6NTac | |
| 13515A-E11 | VGB6 | C57BL/6NTac | |
| 13515A-E9 | VGB6 | C57BL/6NTac | |
