| Gene | Gas2l1 |
| Chromosome | 11 |
| Deletion Start | 5,064,455 |
| Deletion End | 5,060,784 |
| Deletion Size | 3,672 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,064,092 | 5,064,113 | GTGCCTGAAGTGCTCATGTTTG |
| TUP | 5,064,068 | 5,064,091 | AGACAGAGGACCTGGTGCTACGCA |
| TUR | 5,064,047 | 5,064,066 | GCACCACACTCTTCTCGTTC |
| TDF | 5,061,125 | 5,061,146 | GCTCCTCGTCTTCATCACTCAG |
| TDP | 5,061,100 | 5,061,121 | CCTGGGTGGCAAATGTGGCCAA |
| TDR | 5,061,021 | 5,061,040 | TGCCCTCATCGGAAGAACTG |
PCR Primers
| Primer | Sequence |
| SU | ATGGCATTTCTTCCTCGTGG |
| SD | GAGCATTGAGGTCTGGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13517B-G4 | VGB6 | C57BL/6NTac | |
| 13517B-G5 | VGB6 | C57BL/6NTac | |
| 13517C-E4 | VGB6 | C57BL/6NTac | |
| 13517C-H10 | VGB6 | C57BL/6NTac | |
| 13517C-F6 | VGB6 | C57BL/6NTac | |
