13518 Gdf5 Status: ES cell colonies screened / QC no positives
| Gene | Gdf5 |
| Chromosome | 2 |
| Deletion Start | 155,945,052 |
| Deletion End | 155,941,515 |
| Deletion Size | 3,538 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,944,495 | 155,944,515 | GGAGGTAACAGCAGCGTGAAG |
| TUP | 155,944,469 | 155,944,490 | AGGCTGGCCTGGCCAACACCAT |
| TUR | 155,944,435 | 155,944,455 | CCTCTCACCTTGCCCTTTGTC |
| TDF | 155,941,863 | 155,941,881 | AAGCGACCCAGCAAGAACC |
| TDP | 155,941,840 | 155,941,861 | CAAGGCTCGCTGCAGTCGCAAG |
| TDR | 155,941,808 | 155,941,828 | CAGCCCATGTCCTTGAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AAGCCCTCAGTCAGTTGTGC |
| SD | ACCAGGCACAAAGGTGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
