| Gene | Glrx5 |
| Chromosome | 12 |
| Deletion Start | 105,032,703 |
| Deletion End | 105,040,525 |
| Deletion Size | 7,823 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,032,898 | 105,032,916 | AACGCCGTGGTGCAGATTC |
| TUP | 105,032,923 | 105,032,944 | TGCACGGTGTTCGCGACTATGC |
| TUR | 105,032,948 | 105,032,967 | GGTCGTCCAGCACGTTGTAG |
| TDF | 105,039,718 | 105,039,738 | TTCCGTCCTTATCAGGCTCTG |
| TDP | 105,039,739 | 105,039,762 | CAGAGCCCATCCAGGGAGCTGAGA |
| TDR | 105,039,769 | 105,039,791 | GCCTATGGGACAAGACTCCTAAC |
PCR Primers
| Primer | Sequence |
| SU | CGATTTGTCCAATCAACACG |
| SD | GCAGCTAGTTCTCCACGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13520A-C4 | VGB6 | C57BL/6NTac | |
| 13520A-E7 | VGB6 | C57BL/6NTac | |
| 13520A-F3 | VGB6 | C57BL/6NTac | |
| 13520A-F11 | VGB6 | C57BL/6NTac | |
| 13520A-G1 | VGB6 | C57BL/6NTac | |
