| Gene | Mettl21d |
| Chromosome | 12 |
| Deletion Start | 69,582,983 |
| Deletion End | 69,577,924 |
| Deletion Size | 5,060 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,582,120 | 69,582,143 | GCACAATTCAGGATAGGCATACAG |
| TUP | 69,582,092 | 69,582,117 | CTGCCTGTGCGAGGTGGTTGAGAAAA |
| TUR | 69,582,071 | 69,582,091 | CTCCAGTAGAATGCACGCAAG |
| TDF | 69,578,579 | 69,578,599 | TGTGCGTGCTTTACAAGTCTG |
| TDP | 69,578,554 | 69,578,577 | TGTCCTGCACACTTCTGCATCCGG |
| TDR | 69,578,527 | 69,578,546 | GGGAAACGCTGTGCTGAATG |
PCR Primers
| Primer | Sequence |
| SU | TAGCTGCCTAGATCTATCGC |
| SD | AATGTACTGAGCCAACCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13522A-A2 | VGB6 | C57BL/6NTac | |
| 13522A-B11 | VGB6 | C57BL/6NTac | |
| 13522A-A9 | VGB6 | C57BL/6NTac | |
| 13522A-F12 | VGB6 | C57BL/6NTac | |
