| Gene | Gng13 |
| Chromosome | 17 |
| Deletion Start | 25,718,704 |
| Deletion End | 25,719,072 |
| Deletion Size | 369 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,718,745 | 25,718,766 | CCTCAAGTACCAACTGGCCTTC |
| TUP | 25,718,768 | 25,718,795 | AGAGGGAGATGTCGTCCAAGACCATCCC |
| TUR | 25,718,819 | 25,718,838 | TGGCTGGGCAGGTAAATGAC |
| TDF | 25,718,971 | 25,718,993 | TGAACCCAGACCTGATGAAGAAC |
| TDP | 25,718,997 | 25,719,021 | CCTTGGGTAGAGAAGGCCAAGTGCA |
| TDR | 25,719,023 | 25,719,043 | GTGGGTCAGGCTCATAGGATG |
PCR Primers
| Primer | Sequence |
| SU | ACGGAAAGTTTGCTCTGCAG |
| SD | TTTCTGCTAGCCTGAGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13525A-A1 | VGB6 | C57BL/6NTac | |
| 13525B-A10 | VGB6 | C57BL/6NTac | |
| 13525B-D12 | VGB6 | C57BL/6NTac | |
| 13525B-E12 | VGB6 | C57BL/6NTac | |
| 13525B-G5 | VGB6 | C57BL/6NTac | |
