| Gene | Gng3 |
| Chromosome | 19 |
| Deletion Start | 8,838,649 |
| Deletion End | 8,838,133 |
| Deletion Size | 517 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,838,587 | 8,838,608 | AGCACGCAAGATGGTGGAACAG |
| TUP | 8,838,561 | 8,838,585 | TTAAGATTGAGGCCAGCTTGTGCCG |
| TUR | 8,838,540 | 8,838,560 | AGGGTCGCACCCACCTTTATC |
| TDF | 8,838,350 | 8,838,373 | TCTCAAAGGGCACTAGAAACAAAA |
| TDP | | | AGTCTATTGGAGAAGGAACTGCAGTCCC |
| TDR | 8,838,280 | 8,838,303 | CAGCCAGGAAGGGATACATTAACC |
PCR Primers
| Primer | Sequence |
| SU | GATGTGATTTCATCAGGGTGG |
| SD | ATCCTCTTTCCCAAGAACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13526A-B2 | VGB6 | C57BL/6NTac | |
| 13526A-B10 | VGB6 | C57BL/6NTac | |
| 13526A-C3 | VGB6 | C57BL/6NTac | |
| 13526A-E3 | VGB6 | C57BL/6NTac | |
| 13526A-F8 | VGB6 | C57BL/6NTac | |
