| Gene | Gngt2 |
| Chromosome | 11 |
| Deletion Start | 95,845,060 |
| Deletion End | 95,845,625 |
| Deletion Size | 566 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,845,107 | 95,845,129 | GCTGAAGAAGGAAGTGAAGAACC |
| TUP | 95,845,132 | 95,845,155 | CGTGATCTGGTGAGCCTGCAGTCC |
| TUR | 95,845,207 | 95,845,229 | CCAGCCCAGTAATACATCCTGTG |
| TDF | 95,845,392 | 95,845,411 | TCATCTCTACCCTGCCCATC |
| TDP | 95,845,413 | 95,845,438 | TCCTCCGATTGCCAAGTCATGGATTC |
| TDR | 95,845,510 | 95,845,532 | CCTGCTTGGGCCTCTACATAATC |
PCR Primers
| Primer | Sequence |
| SU | CCCGGACAGGACCTAGAGAC |
| SD | AGAAGACATTTGCCACCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 141 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13528A-B2 | VGB6 | C57BL/6NTac | |
| 13528A-B4 | VGB6 | C57BL/6NTac | |
| 13528A-C3 | VGB6 | C57BL/6NTac | |
| 13528A-C11 | VGB6 | C57BL/6NTac | |
| 13528A-D12 | VGB6 | C57BL/6NTac | |
| 13528A-F3 | VGB6 | C57BL/6NTac | |
