| Gene | Golga2 |
| Chromosome | 2 |
| Deletion Start | 32,288,316 |
| Deletion End | 32,306,933 |
| Deletion Size | 18,618 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,288,883 | 32,288,900 | AGTGCGGAGACGCTGTAG |
| TUP | 32,288,902 | 32,288,931 | CGCCCGCATTCCCTTAACTTAGCATTAGTG |
| TUR | 32,288,959 | 32,288,982 | CCAAACCAAACCAATCCATGTCAG |
| TDF | 32,306,594 | 32,306,615 | GACGAGGTGAAGATCATGGTTG |
| TDP | 32,306,620 | 32,306,642 | AAAGACTGGCGGCCAAAGCCTCA |
| TDR | 32,306,679 | 32,306,701 | AAAGATAAGGGTGGCTGAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | GGCTTAAACTCGGAAGGGTC |
| SD | GCCGGTGAAAACTTAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13529A-C4 | VGB6 | C57BL/6NTac | |
| 13529A-C6 | VGB6 | C57BL/6NTac | |
| 13529A-C9 | VGB6 | C57BL/6NTac | |
| 13529A-F5 | VGB6 | C57BL/6NTac | |
| 13529A-B2 | VGB6 | C57BL/6NTac | |
