| Gene | Slc52a2 |
| Chromosome | 15 |
| Deletion Start | 76,539,476 |
| Deletion End | 76,541,168 |
| Deletion Size | 1,693 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,540,238 | 76,540,258 | CCCGACATCTGAACCCACAAC |
| TUP | 76,540,262 | 76,540,286 | TACAGGGCTTCGGGTAGAAACACCA |
| TUR | 76,540,291 | 76,540,312 | TCCTCTTCCTCTTCCTCCTCTG |
| TDF | 76,540,368 | 76,540,388 | CCAGACCCTAAGGCCCATCAG |
| TDP | 76,540,390 | 76,540,413 | TGTTCTCTTCCCGAAGTGCCTGTC |
| TDR | 76,540,441 | 76,540,460 | CAGCACGCCATTGGTCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GACTGGCTCTTTCGCTCATC |
| SD | CACTAGGCGAGTGGAGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13532A-A6 | VGB6 | C57BL/6NTac | |
| 13532A-D11 | VGB6 | C57BL/6NTac | |
| 13532A-H9 | VGB6 | C57BL/6NTac | |
| 13532A-H6 | VGB6 | C57BL/6NTac | |
| 13532A-D2 | VGB6 | C57BL/6NTac | |
