| Gene | Tpra1 |
| Chromosome | 6 |
| Deletion Start | 88,907,960 |
| Deletion End | 88,911,989 |
| Deletion Size | 4,030 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,908,622 | 88,908,641 | TCAGTCCACCATTCCTCCAC |
| TUP | 88,908,644 | 88,908,670 | AGGTAATGTCTTTGCAGCAAGCTCACC |
| TUR | 88,908,684 | 88,908,702 | GCCCACCAAAGGCTGAGAC |
| TDF | 88,911,853 | 88,911,876 | GGGTGGCCTACTTAGATGACATTG |
| TDP | 88,911,879 | 88,911,898 | TCCATGCCCTGCCACACGGG |
| TDR | 88,911,902 | 88,911,922 | TCGCTGTCTGTGCTGTTGATG |
PCR Primers
| Primer | Sequence |
| SU | GCCTATCATTGCTCTCTGGC |
| SD | CTCAGCTCCACAGAACTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13533A-D10 | VGB6 | C57BL/6NTac | |
| 13533A-F6 | VGB6 | C57BL/6NTac | |
| 13533A-G6 | VGB6 | C57BL/6NTac | |
