| Gene | Gpsm3 |
| Chromosome | 17 |
| Deletion Start | 34,590,225 |
| Deletion End | 34,591,320 |
| Deletion Size | 1,096 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,590,619 | 34,590,640 | GGTCCACTAACCATCACACCTG |
| TUP | 34,590,641 | 34,590,662 | TTGGTCCCTAGCTCGTCCACCA |
| TUR | 34,590,692 | 34,590,712 | CCCAGAGGCTATGGCAGAATG |
| TDF | 34,590,910 | 34,590,930 | AACAGCTCTACAGCACCATCC |
| TDP | 34,590,935 | 34,590,958 | TCACCAGGTAAGGCACATCCCACC |
| TDR | 34,590,988 | 34,591,007 | CAGAAGGCCAGCTAGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGCTCTGGAGATGAGC |
| SD | CAGACAGACTCAGCAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13534A-A4 | VGB6 | C57BL/6NTac | |
| 13534A-H8 | VGB6 | C57BL/6NTac | |
| 13534A-B8 | VGB6 | C57BL/6NTac | |
| 13534A-D8 | VGB6 | C57BL/6NTac | |
| 13534A-G9 | VGB6 | C57BL/6NTac | |
| 13534A-G10 | VGB6 | C57BL/6NTac | |
