| Gene | Gpx1 |
| Chromosome | 9 |
| Deletion Start | 108,339,316 |
| Deletion End | 108,340,211 |
| Deletion Size | 896 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,339,466 | 108,339,486 | CGGGACTACACCGAGATGAAC |
| TUP | 108,339,490 | 108,339,512 | CTGCAGAAGCGTCTGGGACCTCG |
| TUR | 108,339,530 | 108,339,550 | CGAACTGATTGCACGGGAAAC |
| TDF | 108,340,065 | 108,340,082 | CGCCGCTTTCGTACCATC |
| TDP | 108,340,088 | 108,340,115 | CGAACCTGACATAGAAACCCTGCTGTCC |
| TDR | 108,340,121 | 108,340,139 | GCCTTAGGAGTTGCCAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTGTGCGTGAGCTGCTTTAG |
| SD | GTCTGCCCTTGTCTACTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13535A-A5 | VGB6 | C57BL/6NTac | |
| 13535A-A7 | VGB6 | C57BL/6NTac | |
| 13535A-A10 | VGB6 | C57BL/6NTac | |
| 13535A-B5 | VGB6 | C57BL/6NTac | |
| 13535A-E12 | VGB6 | C57BL/6NTac | |
| 13535A-D11 | VGB6 | C57BL/6NTac | |
