Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

13536 Gpx4 Status: ES cell colonies screened / QC positives

Gene	Gpx4
Chromosome	10
Deletion Start	80,053,635
Deletion End	80,056,221
Deletion Size	2,587
Genome Build	38

MGI ID	MGI:104767
NCBI Gene ID	625249
BAC ID	365H4
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	80,054,286	80,054,305	GAGTTCCTGGGCTTGGTGTG
TUP	80,054,319	80,054,342	CCCTGCCGTCCTCTTCCCTGGTAG
TUR	80,054,406	80,054,426	GGTGGAGGAGTCTCAAGGTTC
TDF	80,055,688	80,055,707	GCTGGGAGTGTAGCCCATTC
TDP	80,055,709	80,055,736	CCAGAAGAGCAGTGCATTCCTCTAACCA
TDR	80,055,746	80,055,767	GCCAGGGAAACTGAAGCTGTAG

PCR Primers

Primer	Sequence
SU	CATGCGCAGTTGCTGATAAC
SD	CCTTAAACAGCCCTGTCCAC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	141 bp	+	+	-
TDF	TDR	80 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
13536A-C5	VGB6	C57BL/6NTac
13536A-B6	VGB6	C57BL/6NTac
13536A-E7	VGB6	C57BL/6NTac