| Gene | Grb7 |
| Chromosome | 11 |
| Deletion Start | 98,450,774 |
| Deletion End | 98,455,047 |
| Deletion Size | 4,274 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,450,977 | 98,450,997 | AGCCAGCTCTGACTGACATAC |
| TUP | 98,450,998 | 98,451,026 | AGGGCTTCTATCCTTCACTCGTTGCCATA |
| TUR | 98,451,035 | 98,451,055 | TGCCTGAAACTCCTCTTCTCG |
| TDF | 98,454,875 | 98,454,897 | GCCTTTACTTCAGCATGGATGAG |
| TDP | 98,454,901 | 98,454,925 | CAGACCCGTTTCACAGACCTGCTGC |
| TDR | 98,454,946 | 98,454,964 | GCAGGATGCCTCGGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | GAGGTACCGAACGACAAAGC |
| SD | CTGCTAGGATTTGGACGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13537A-A5 | VGB6 | C57BL/6NTac | |
| 13537A-D2 | VGB6 | C57BL/6NTac | |
| 13537A-F2 | VGB6 | C57BL/6NTac | |
| 13537A-G11 | VGB6 | C57BL/6NTac | |
