| Gene | Grifin |
| Chromosome | 5 |
| Deletion Start | 140,565,050 |
| Deletion End | 140,563,216 |
| Deletion Size | 1,835 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,564,556 | 140,564,575 | TAACAGACGCCGGAGACATC |
| TUP | 140,564,528 | 140,564,554 | CCTTCCACGTCAAACCTCGATTCTCCA |
| TUR | 140,564,491 | 140,564,511 | CGTCCTCCCTGGAAAGCATTG |
| TDF | 140,563,634 | 140,563,652 | TGCTCCAGTTCCCACATCG |
| TDP | 140,563,611 | 140,563,631 | ACCGACCGCTGGCCACCATCA |
| TDR | 140,563,590 | 140,563,609 | CACTGAGCACTCGCACTCTG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTCCCTACAAAGATGGG |
| SD | ACCACAGTCTCCTGTCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13538A-C1 | VGB6 | C57BL/6NTac | |
| 13538A-H4 | VGB6 | C57BL/6NTac | |
| 13538A-D4 | VGB6 | C57BL/6NTac | |
| 13538A-D8 | VGB6 | C57BL/6NTac | |
| 13538A-E2 | VGB6 | C57BL/6NTac | |
| 13538A-F5 | VGB6 | C57BL/6NTac | |
