| Gene | Gsc2 |
| Chromosome | 16 |
| Deletion Start | 17,915,000 |
| Deletion End | 17,913,615 |
| Deletion Size | 1,386 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,914,478 | 17,914,497 | CGCCACCGTACCATCTTCAG |
| TUP | 17,914,445 | 17,914,467 | CTGCAGGCGCTCGAGGCACTCTT |
| TUR | 17,914,420 | 17,914,440 | TACGTCGGGATACTGGTTCTG |
| TDF | 17,913,692 | 17,913,709 | AAGAACCGCCGGGCAAAG |
| TDP | 17,913,669 | 17,913,691 | TGGCGACACCAAAAGCGTGCTTC |
| TDR | 17,913,636 | 17,913,658 | GTTTTCTTAGTCCCAGGCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | GACCAGGGCTTCAAAGTGAG |
| SD | GGGAGTTAGGCTCTTCGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13539A-B3 | VGB6 | C57BL/6NTac | |
| 13539A-D7 | VGB6 | C57BL/6NTac | |
| 13539A-E8 | VGB6 | C57BL/6NTac | |
| 13539A-F1 | VGB6 | C57BL/6NTac | |
| 13539A-F12 | VGB6 | C57BL/6NTac | |
| 13539A-H8 | VGB6 | C57BL/6NTac | |
