| Gene | Gstt2 |
| Chromosome | 10 |
| Deletion Start | 75,834,283 |
| Deletion End | 75,831,584 |
| Deletion Size | 2,700 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,833,921 | 75,833,940 | TGAAGGCACGCAGTGTTTAG |
| TUP | 75,833,884 | 75,833,913 | AAATTCCGGAGACTTTGCAGGGAAGAGACA |
| TUR | 75,833,851 | 75,833,869 | GGGAACCAGAGGCACAGAG |
| TDF | 75,832,113 | 75,832,135 | CCCTCGGTATCTCTTCGACTTTC |
| TDP | 75,832,085 | 75,832,109 | TCTTTGTCTCTACAGCCCGTGGCTC |
| TDR | 75,832,055 | 75,832,073 | GAGGCCGTCCCTCAAACAG |
PCR Primers
| Primer | Sequence |
| SU | GCAAACGACTACGTCCACAG |
| SD | TGATTGGTTTCTTTGGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13540A-C4 | VGB6 | C57BL/6NTac | |
| 13540A-D9 | VGB6 | C57BL/6NTac | |
| 13540A-E1 | VGB6 | C57BL/6NTac | |
| 13540A-H4 | VGB6 | C57BL/6NTac | |
| 13540A-H1 | VGB6 | C57BL/6NTac | |
| 13540A-D12 | VGB6 | C57BL/6NTac | |
