| Gene | Gyltl1b |
| Chromosome | 2 |
| Deletion Start | 92,370,660 |
| Deletion End | 92,365,248 |
| Deletion Size | 5,413 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,369,952 | 92,369,972 | TGCACCTCCACCTGATAACTG |
| TUP | 92,369,927 | 92,369,950 | TGCCGTAGCCAGAAACATCCTGGA |
| TUR | 92,369,897 | 92,369,915 | GCTGGCACCATCCATGTTC |
| TDF | 92,365,831 | 92,365,848 | TGCTCGCACCTGCTTCTG |
| TDP | 92,365,807 | 92,365,830 | TTACACAGGTACCACGAGTGGCCA |
| TDR | 92,365,777 | 92,365,798 | GCGGGAATAGTCTGTGGATGAG |
PCR Primers
| Primer | Sequence |
| SU | GCTAGTTCGCGATTCTGACC |
| SD | CTTTATTGAAATGTCGCGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13543A-C1 | VGB6 | C57BL/6NTac | |
| 13543A-G5 | VGB6 | C57BL/6NTac | |
| 13543A-F12 | VGB6 | C57BL/6NTac | |
| 13543A-D7 | VGB6 | C57BL/6NTac | |
| 13543A-C11 | VGB6 | C57BL/6NTac | |
| 13543A-E11 | VGB6 | C57BL/6NTac | |
