| Gene | H2-Ke2 |
| Chromosome | 17 |
| Deletion Start | 33,940,084 |
| Deletion End | 33,938,969 |
| Deletion Size | 1,116 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,939,673 | 33,939,695 | TCATCTCATTCACTGCCTCATCC |
| TUP | 33,939,649 | 33,939,671 | CTTCTCAGGAGCTGGCCCTGCTG |
| TUR | 33,939,605 | 33,939,624 | ACAAGCACGGGTCCCAGAAG |
| TDF | 33,939,122 | 33,939,142 | CTCTCCGTTTGCCTCCCTTTG |
| TDP | 33,939,100 | 33,939,121 | CCCTCCTCAGCAAGCGCTACGA |
| TDR | 33,939,052 | 33,939,072 | GTTTCCCTCTGTTGCTCTGAC |
PCR Primers
| Primer | Sequence |
| SU | CATGTTCCAGCATGTATCGG |
| SD | CTGTCATTTCCTGGGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13544A-F2 | VGB6 | C57BL/6NTac | |
| 13544B-G2 | VGB6 | C57BL/6NTac | |
