| Gene | Haghl |
| Chromosome | 17 |
| Deletion Start | 25,785,105 |
| Deletion End | 25,782,926 |
| Deletion Size | 2,180 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,784,262 | 25,784,281 | GACTCACCAGCCCTCTTCTC |
| TUP | 25,784,239 | 25,784,261 | TGGTACCGTGCAGCCTGGATTCA |
| TUR | 25,784,215 | 25,784,237 | GCACAGTGGAATTGGGTAGGTAG |
| TDF | 25,783,615 | 25,783,633 | CCTGCTGTGCCGTAACCTC |
| TDP | 25,783,591 | 25,783,614 | CCCGTGGCCGTTGGTTCTTCTGTG |
| TDR | 25,783,565 | 25,783,585 | ATCCTCGTCATCCCTCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | AGTGCAGTTAAGAGAGGCGG |
| SD | TACCTACTGAGGCACCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13546A-A7 | VGB6 | C57BL/6NTac | |
| 13546A-C5 | VGB6 | C57BL/6NTac | |
| 13546A-D3 | VGB6 | C57BL/6NTac | |
| 13546A-E8 | VGB6 | C57BL/6NTac | |
| 13546A-F9 | VGB6 | C57BL/6NTac | |
| 13546A-F12 | VGB6 | C57BL/6NTac | |
