| Gene | Hcfc1r1 |
| Chromosome | 17 |
| Deletion Start | 23,673,986 |
| Deletion End | 23,675,064 |
| Deletion Size | 1,079 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,674,183 | 23,674,201 | CGTGGTGCTTGAGCTTGTG |
| TUP | 23,674,203 | 23,674,225 | TGAAGCAGGGAAGCCAAGGTCAA |
| TUR | 23,674,243 | 23,674,265 | CCTTCCTCTCCCAGGCTATACAC |
| TDF | 23,674,764 | 23,674,784 | GCTTCTAGCCAACTTGTGCTG |
| TDP | 23,674,789 | 23,674,810 | CCATCTTTGGCTCCAGGCGACC |
| TDR | 23,674,830 | 23,674,849 | AGAAGGCTGGCTTGGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGGCACTGTGCATACACTCG |
| SD | CACACATTCTCAATGCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13547A-B8 | VGB6 | C57BL/6NTac | |
| 13547A-E10 | VGB6 | C57BL/6NTac | |
